Answer questions from the word files, some are MCQ and some requires with workout and unit.
questions_biology.docx
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Question 1
Match the fixative to the cellular component to be visualized.
i) To image microfilaments, fix with
a) Formaldehyde b) Glue
c) Methanol
Answer:
ii) To image microtubules, fix with
a) Formaldehyde b) Glue
c) Methanol
Answer:
Question 2
You require 400 µl of antibody solution to stain the slide and the antibody to be used need to be
diluted 1/2000. How much stock antibody solution should you add to the blocking solution?
Answer:
Question 3
You are staining cells for DNA, microtubules and the Golgi. Match the secondary antibodies to
the primary antibodies.
i) DAPI
Answer:
ii) Rabbit anti-Golgin 96
Answer:
iii) Mouse monoclonal to alpha tubulin
Answer:
a)
b)
c)
d)
Sheep anti-DAPI IgM Azure Blue
Donkey anti-rabbit IgG Taxas Red
Goat anti-mouse IgG Alexa Fluor 488
None needed
Question 4
You diluted your antibodies 1 in 100. The stocks we gave you were diluted 1 in 5. What was the
net dilution?
Answer:
Question 5
The actin antibody is supplied at 1 mg per ml and you dilute 1/500. What is the final
concentration that you use?
Answer:
Question 6
How does cold treatment affect the cytoskeleton?
a) The intermediate filaments depolymerize but the microtubules and microfilaments are
unaffected
b) The microtubules depolymerize but the microfilaments and intermediate filaments are
unaffected
c) The microtubules and intermediate filaments depolymerize but the microfilaments are
unaffected
d) The intermediate filaments and microfilaments depolymerize but the microtubules are
unaffected
e) The microfilaments depolymerize but the microtubules and intermediate filaments are
unaffected
f) All three components depolymerize
g) The microtubules and microfilaments depolymerize but the intermediate filaments are
unaffected
Answer:
Question 7
Microtubules can also be depolymerized by treatment with
a)
b)
c)
d)
e)
Taxol
Cytochalasin D
Nocodazole
Phalloidin
Colchicine
Answer:
Question 8
Which of these is not used as a fixative?
a)
b)
c)
d)
e)
Ethanol
Acetone
Formaldehyde
Paraformaldehyde
Methanol
Answer:
Question 9
Match the images to the cytoskeleton.
i)
a) Actin
b) Intermediate filaments
c) Microtubules
Answer:
ii)
a) Actin
b) Intermediate filaments
c) Microtubules
Answer:
…
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