Expert answer:BIO 320 – How much DNA has been extracted prior

  

Solved by a verified expert:We need to know how much DNA has been extracted prior to amplifying the 15 loci of interest. A technique called quantitative real-time polymerase chain reaction (qRT-PCR) is available to help us. Please explain how this works and why an internal standard is included in each reaction. Please touch on the why you need to make a standard curve with each quantification run.

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