Description You must have an introduction stating what column and TLC chromatography is. State the purpose of the experiment. Summarize in your own words the experimental procedures carried out in the three videos. I strongly suggest you summarize the experimental procedures from the videos as instructions that you would find in a lab manual or a cooking recipe ANSWER THE POST LAB QUESTIONS FOR THIS EXPERIMENT Column and TLC Post lab Questions Which of pigment molecule was the most nonpolar from the handout and videos? Which of your pigment molecules was the most polar from the handout and videos? Why should you not use ink on the stationary phase to mark your pigment placement? In the TLC part of the experiment, why must the spot be applied to the TLC plate above the level of the developing solvent? What will be the result of applying too much compound to a TLC plate? What are some applications of TLC in an organic chemistry laboratory? Mention at least 3. What are some applications of using column chromatography in an organic chemistry laboratory? Mention at least 2 What would happen if the solvent system used to develop the TLC in the experiment was 70% acetone/30% hexanes? A compound travelled 6.0 cm on a TLC plate while the solvent front advanced 9.0 cm. What is the Rf value? Show calculations. The pigments from spinach used in this experiment were highly colored, so it was easy to see their progress as they moved down the column and up the TLC plate during elution. Most organic compounds, however, are colorless in solution. Suggest two ways of how you could detect spots on a TLC plate if the compounds in the sample being separated are colorless.
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