Expert answer:What happens if the wrong primers are used in PCR

  

Solved by a verified expert:1) What happens if the wrong primers are used in PCR? Will the reaction proceed or halt at a certain point and what stage of the process does it impair? What are the potential consequences of this? Be very specific in your answer.2) What happens if only 3 of the 4 dNTP’s are used in PCR? Will the reaction proceed or halt at a certain stage of the reaction and what are the potential consequences of this?3) What are the roles of the components of the PCR master mix (i.e. Taq polymerase, reaction buffer 1X TAE, MgCl2, and the four major dNTPs). Be very specific as to their function in the reaction and how they could affect the specificity, accuracy/fidelity, and the yield of the DNA.

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