Solved by a verified expert:TAKE-HOME EXTRA CREDIT
These questions are open-note, open-book, chat with all of your
colleagues questions. I ask only a couple things:
1. Use your own
words. I will pick up copy-and-paste, and it will make me cranky. You won’t
like me cranky.
2. Cite any and
all references you use. I don’t care about format, so long as it contains all
of the relevant information. Questions? Email me.
Nicole and I will set up a drop-box on D2L for you to submit these
answers by 5PM Wednesday, April 29.
You don’t have to do all of them – you can pick and choose.
I will post these questions and all of the relevant information on
D2L after the exam.
1. You have cloned a region
of DNA that contains a BINDING SITE (and ONLY a binding site!) for a regulatory
protein from a prokaryotic operon. You put this clone into a high copy plasmid
and introduce it into a cell that is wild type for the regulation you are
studying. (5 pts)
A) What effect would this have if
the binding site is involved in positive regulation and why?
B) What effect would this have if
the binding site is involved in negative regulation and why?
2. You have received two
plasmids in the mail, pHS1 and pEC1. (5 pts)
pHS1 is a plasmid
that contains a 7 kb EcoRI fragment
from the human genome
pEC1 is a plasmid
that contains a 7 kb EcoRI fragment
for the E. coli genome
Unfortunately, the tube labels
washed off in transit. Describe how you
would determine which tube contains which fragment. Note that you want to
tackle this without doing any DNA sequencing.
3. Describe the process of producing a cDNA library for genes ONLY
expressed in one tissue of your eukaryotic organism of choice. Include all
relevant enzymes. (10 pts)
4. Describe at least two DNA sequencing methods, including
chemistry, positives, and minuses of each technique. (10 pts)
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