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UMUC Biology 102/103
Lab3:Cell Structure and Function
INSTRUCTIONS:

·
On your own and
without assistance, complete thisLab 3Answer Sheet
electronically and submit it via theAssignments Folder by the date listed in theCourse Schedule (underSyllabus).
·
To conduct your
laboratory exercises, use the Laboratory Manual located under Course Content.
Read the introduction and the directions for each exercise/experiment carefully
before completing the exercises/experiments and answering the questions.
·
Save your Lab 3
Answer Sheet in the following format:
LastName_Lab3 (e.g., Smith_Lab3).
·
You should
submit your document as a Word (.doc or .docx) or Rich Text Format
(.rtf) file for best compatibility.

Pre-Lab
Questions
1.
Identify
three major similarities and differences between prokaryotic and eukaryotic
cells.

2.
Where
is the DNA housed in a prokaryotic cell? Where is it housed in a eukaryotic
cell?

3.
Identify
three structures which provide support and protection in a eukaryotic cell.

Experiment 1: Cell Structure and Function

Label
each of the arrows in the following slide image:

Post-Lab Questions

1.
What
is the difference between the rough and smooth endoplasmic reticulum?

2.
Would
an animal cell be able to survive without mitochondria? Why or why not?

3.
What
could you determine about a specimen if you observed a slide image showing the
specimen with a cell wall, but no nucleus or mitochondria?

4.
Hypothesize
why parts of a plant, such as the leaves, are green, but other parts, such as
the roots, are not. Use scientific reasoning to support your hypothesis.

Experiment
2: Osmosis – Direction and Concentration Gradients
Data Tables and Post-Lab Assessment
Table 3: Sucrose Concentration vs. Tubing Permeability

Table 3: Sucrose
Concentration vs. Tubing Permeability

Band
Color

% Sucrose
in Beaker

%
Sucrose in Bag

Initial
Volume (mL)

Final
Volume (mL)

Net
Displacement (mL)

Yellow

Red

Blue

Green

Hypothesis:

Post-Lab Questions
1.
For
each of the tubing pieces, identify whether the solution inside was
hypotonic, hypertonic, or isotonic in comparison to the beaker solution in
which it was placed.

2.
Which
tubing increased the most in volume? Explain why this happened.

3.
What
do the results of this experiment this tell you about the relative tonicity
between the contents of the tubing and the solution in the beaker?

4.
What
would happen if the tubing with the yellow band was placed in a beaker of
distilled water?

5.
How
are excess salts that accumulate in cells transferred to the blood stream so
they can be removed from the body?

6.
If
you wanted water to flow out of a tubing piece filled with a 50% solution, what
would the minimum concentration of the beaker solution need to be? Explain your
answer using scientific evidence.

7.
How
is this experiment similar to the way a cell membrane works in the body? How is
it different? Be specific with your response.