Solved by a verified expert:Biology 302 Molecular and General GeneticsSummer 2013 Exam 6FORM 0__________________________________________Name, please print legiblyINSTRUCTIONS: in your name in the above blank.On the Scantron sheet, fill in your name and student ID number.On the Scantron sheet, fill in the exam form number shown above in box K.Read the statements below, sign your name, and then tear off this cover sheet.Turn in this cover sheet and Scantron sheet. You can keep your question sheets.The answer Key will be posted in the glass case outside the elevators in the Biologybuilding on the first floor.7. Keep your answer sheet covered.Note: The use of a cell phone during an exam in any manner is expressly prohibited andwill result in a zero on the exam and a report filed to the UMBC ACC with nowarning.I pledge that I will neither seek help nor offer help to any other student while taking this exam. Iwill make every effort to assure that no other student can see my answers. I also state that I haveno aids, such as crib sheets and that I know of none used by other students.I understand that any confirmed case of cheating on an this exam will be dealt with throughUMBCs Academic Conduct Committee as described in the Policy for Academic Misconduct inUndergraduate Courses. Any and all instances of academic misconduct will be reported to thecommittee. Penalties include, but are not limited to, a grade of F in the class.__________________________________________Signature and Date Summer 2013 BIOL 302EXAM 6 Form 01. The DNA sequence below contains a palindromic sequence consisting of:A G G A G C T T C T T C C T C G A A G A a.b.c.d.e.One palindrome of 4 basesTwo palindromes of 4 bases eachOne palindrome of 6 basesOne palindrome of 4 bases and one palindrome of 6 basesThere is no palindromic sequence longer than three basesUse the information below to answer the next two questions.To the right is a map of the vector pUC19. Imagine you were to clone(insert) a copy of the human insulin gene into this plasmid obtained byperforming RT-PCR on an mRNA sample taken from humanpancreatic beta cells. You then transform a strain of E. coli that isampS and lac- with the construct you have made.MCS2. In this vector, what was the MCS used for?a. Selection of transformantsb. Screening for conjugatesc. Insertion of DNAd. Transformation of host cellse. Production of pod people3. When you succeed in putting the insulin gene into the E. coli cells, the resulting cells willbe:a. AmpS, lacb. AmpS, lac+c. AmpR, lacd. AmpR, lac+e. Why would E. coli need insulin?4. A cDNA library:a. Does not contain regulatory sequencesb. Requires the use of reverse transcriptasec. Contains intron sequencesd. More than one of the abovee. Can be replaced with iTunes for many people Summer 2013 BIOL 302EXAM 6 Form 05. Which of the following will be incorporated into the DNA produced by PCR?a. dNTPs, but not primers will be incorporated into the new DNAb. Primers, but not dNTPs will be incorporated into the new DNAc. Both dNTPs and primers will be incorporated into the new DNAd. Neither dNTPs nor primers will be incorporated into the new DNAe. I can still see the afterimage from the fireworks, why are you bothering me?5A<Target>C<Target>BD6. Above is a melted fragment of DNA with a target you wish to amplify by PCR. Wherewould you design the primers to anneal?a. A and Bb. B and Cc. B and Dd. A and De. None of the above7. If you want to put DNA in a bacterium, and a plasmid wont hold enough, you couldconsider using a:a. Cosmidb. BACc. YACd. Two of the abovee. All of the above8. Chain terminators are:a. Altered nucleotidesb. Used for PCRc. Used for southern blottingd. More than one of the above9. Which type of sequencing includes the use of chain-terminators?a. Sanger sequencingb. Pyro-sequencingc. Both Sanger sequencing and pyro-sequencingd. Neither sanger sequencing nor pyro-sequencinge. What does sequencing have to do with DNA? Summer 2013 BIOL 302EXAM 6 Form 010. In a southern blot, the probing is done with:a. Antibodiesb. A digested vectorc. Single stranded DNA or RNAd. Restriction enzymese. None of the above11. A two channel gene array is performed comparing human male DNA, which is labeledgreen, and human female DNA, which is labeled red. An active Y-linked gene would beexpected to fluoresce:a. Redb. Greenc. Yellowd. Black12. Three individuals underwent RFLP analysis to test for theS allele by digestion of some of their DNA with MstII,which cuts the gene approximate 200 bp downstream fromthe 5 end. To the right is a southern blot showing where inthe gel the DNA fragments of the three individuals islocated. If a single probe that bound the first 20 bp of thegene (5 end) was used, which bands would you see in theheterozygote?a. The 0.2 kb onlyb. The 1.1 kb onlyc. The 0.2 kb and 1.1 kb bandsd. The 0.2 kb and 1.3 kb bandse. The 1.1 kb and 1.3 kb bandsABC1.3 kb1.1 kb0.2 kb13. Exposing the larvae of normal fruit flies to sodium metaborate results in the developmentof greatly reduced eye size in a manner like that caused by the autosomal recessivemutation eyeless. This is a typical example of:a. Parental Determinationb. Phenocopyc. Genomic Imprintingd. Simple Mendelian Inheritancee. Anticipation Summer 2013 BIOL 302EXAM 6 Form 0DaveSteveNicko14. Which student has the fewest BamHI sites?a. Bruceb. Nickoc. Steved. Davee. More than one of the aboveBruceOccasionally, a random student is kidnapped from the commonsand a sample of the DNA is taken for testing before they arereleased. When a particular 10 kb stretch of DNA from10 kbfour students is digested with BamHI and run on a gel.9 kbThe gel is shown to the right.5 kb3 kb2 kb1 kb15. Which student has the most BamHI sites?a. Bruceb. Nickoc. Steved. Davee. More than one of the above16. Spinocerebellar ataxia (SCA-1) shows differences in gene action dependent upon whichparent the allele was inherited from. This is called:a. Genomic Imprintingb. Haplotypingc. Anticipationd. Parental Determinatione. Phenocopy17. What makes Huntingtons a poor candidate for gene therapy?a. The disease results from a single geneb. Its relatively easy to remove and replace the target cellsc. The mutation is recessived. The mutation is dominante. Two of the above are correct18. An adult individual homozygous for HPFH will produce which type of hemoglobin?a. HbA hemoglobinb. HbS hemoglobinc. HbGower hemoglobind. HbF hemoglobine. More than one of the above Summer 2013 BIOL 302EXAM 6 Form 0Below are maps of the wild type and mutant versions of the CFTR gene, which is responsible forcystic fibrosis, along with the binding location of a probe that can be used to investigate it. Thecut sites by a restriction enzyme (labeled R) are also indicated. Also shown is a Southern Blot ofparents and a fetus that was tested.FatherFetusMotherRRWT CFTRCFTR probeRRRMutant CFTR700 bp300 bp19. What size band(s) would be visible in samples from individuals who are heterozygous forcystic fibrosis?a. 300 bpb. 700 bpc. 1000 bp and 700 bpd. 1000 bp and 300 bpe. 1000 bp, 700 bp, and 300 bp20. Cystic fibrosis is a recessive disease. If we ignore the possibility of reduced penetranceand other such phenomena, will the fetus have the disease when born?a. Nob. There is a 25% chancec. There is a 50% chanced. Yese. It is impossible to tell21. What is the most likely nature of the CFTR probe when it is being used?a. Double stranded DNAb. Single stranded DNAc. Double stranded RNAd. Single stranded RNAe. Surgical stainless steel Summer 2013 BIOL 302EXAM 6 Form 022. Huntingtons is a tri-base duplication caused disease like Fragile-X or SCA-1. WithHuntingtons, the length of the duplication affects the disease such that a longerduplication results in:a. Less severe symptomsb. More severe sever symptomsc. No disease occurrenced. A later age of onsete. An earlier age on onset23. Like Huntingtons disease, the severity and age of onset of Myotonic Dystrophy, anautosomal dominant multi-system disease, results from a tribase tandem repeat. Childrenof individuals with the condition typically inherit longer repeats, though much morecommonly and severely when inherited from their mother than their father. Which of thefollowing phenomena does Myotonic Dystrophy demonstrate?a. Gene amplificationb. Genomic imprintingc. Phenocopyd. Histone modificatione. None of the above24. Which of the following pre-natal tests is performed by taking a sample of placentaltissue, often by using a flexible catheter rather than a needle?a. Amniocentesisb. Fetal ultrasoundc. Free fetal DNA testingd. Chorion villus samplinge. Cell culturing25. Prenatal testing can be done to identify a developing fetus for an identified gene. Whichcurrently approved procedure can be done at the earliest point in the pregnancy?a. Chorion villus samplingb. Transgenic analysisc. Amniocentesisd. Tricorder scanninge. More than one of the above Summer 2013 BIOL 302EXAM 6 Form 026. Which of the following sequencing methodologies use chain terminators?a. Sanger sequencingb. Ion torrent sequencingc. Pyro sequencingd. Illumina sequencinge. More than one of the above use chain terminators27. Early attempts by NIH to treat ADA with gene therapy were ultimately unsuccessful,while later attempts by the San Raffaele Telethon Institute for Gene Therapy in Italy weresuccessful starting with Salsabil in 2001. Why did the Italian group have a moresuccessful outcome?a. Because ADA is a dominant diseaseb. The Italian group used older patients who had been receiving drug therapy priorto the gene therapyc. The NIH group used microinjectiond. They went after a different diseasee. The Italian group treat bone marrow cells