Solved by a verified expert:Lactose transporter of E. coli is a well-studied proton driven cotransporter. Ittransports one lactose molecule together with one proton into the cell. The pH out of thecell is 5.0 and 7.5 inside the cell. The membrane potential of the E. coli cell membrane is60 mV (inside negative). What is the free-energy change for transporting one mole of H+into the bacteria at 25 ºC? Can this transporter work against a lactose gradient of 5,000?