Solved by a verified expert:Final
Applied Lab Project
Addresses
course outcomes 1-5:
apply the scientific method to
scientific investigations
state a scientific hypothesis
and design a basic experiment
conduct an experiment, make
observations, and collect data
use knowledge of biological
principles to correctly interpret qualitative and quantitative information
use critical analysis to draw
conclusions
This
is the culminating assessment in BIOL 102. It is designed to assess your
ability to apply the principles of the scientific method. For this project, you
will complete the activity below. Make sure to address all points (questions)
associated with the activity.
Final
Applied Lab Project
Design
an experiment in which you will test the effect of an acidic fluid on enzymatic
activity. (Recall: enzymes are proteins.) To complete this project, it may be
useful for you to review theScientific Method Tutorial (found the Course
Content section of the classroom under the Science Learning Center link) and
theScientific Method lab (Lab 1), so that you can better understand
how to design an experiment. It may also be helpful for you to review Lab 4
(Enzymes). As you review Lab 4, you will be reminded that there are several
factors that impact enzymatic activity: pH, temperature, and amount of reagent.
Feel free to refer to observations and information from Lab 4 as you complete
the questions required in the Final Applied Project (see the questions below).
As
you design your experiment for this project, please remember that you are
trying to examine how an acidic fluid will modify the outcome of an enzymatic
reaction. To successfully complete this project, you will need to identify the
question(s) being asked in your experiment and the hypothesis that you are
testing. In your experimental design, you must clearly explain what you
are doing. That means that you will need to identify the enzyme and the acid,
as well as explain your experimental protocol (this information will help you
to answer question 2). You must also
thoroughly explain how the addition of the acidic fluid impacted the overall
reaction process (this information will help you to answer question 4).
Hint:
Keep in mind that the acid will change the environmental conditions of the
experiment (for example, a low pH value could change the shape of the active
site on the enzyme protein), without directly participating in the reaction.
Lab
Materials
You
may need the following, depending on your experimental design.
Materials
in your lab kit:
none
Additional
materials you may need:
plastic beakers or cups
hydrogen peroxide
solution (from Lab 4)
yeast (from Lab 4)
sample of fresh meat, about 1
cm cubed in size (unprocessed and uncooked, e.g., liver, steak, fish, or
poultry—particularly organs)
sample of fresh vegetable,
about 1 cm cubed in size (e.g., potatoes or other root vegetables)
a pen for labeling the beakers
or cups
a ruler with centimeter
markings
Remember,
the goal of this project is for you to evaluate the effect of an acid on
enzymatic activity. As part of this assignment, you must identify your source
of enzyme and the acid used. As you work though this project, you must also
make sure that the following questions/points are addressed:
1.
What question are you asking? Clearly state your hypothesis.
2.
Design an experiment. Provide a detailed account of the materials and methods
used to conduct the experiment. Also include the methods for data collection
and analysis.
3.
Conduct the experiment and record your results below. What did you observe?
Which samples showed bubbling?
4.
Use your knowledge about factors that effect enzymatic activity to interpret
your results. It may be necessary for you to refer to your lab manual and/or
other resources. What effect did the acid have on the enzyme?
5.
Was your hypothesis supported? What is your conclusion? Explain your answer.
6.
Cite all references used to complete your Final Applied Project.
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