Solved by a verified expert:Electrophoresis (DNA) Lab ReportGeneral Concepts1. How does gel electrophoresis sort DNA fragments?2. If each individual has such a small amount of DNA, how do the bands on the gel containenough DNA to be visible?3. Many genes only have a few possible alleles. For example, humans only have a few eye colorsand only four blood types. How can DNA tests definitively identify individuals when many menhave brown eyes or type A blood?Experiment-Specific QuestionsLab Part 3: Analyzing the Results1. List the distances traveled (in mm) for the bands in the DNA Ladder in the table below.Remember, smaller fragments travel farther than longer ones, so the top-most band will be the1,000 bp fragments while the bottom-most band will be the 50 bp fragments.DNA LadderBand Distance (mm)50 bp100 bp150 bp200 bp250 bp300 bp400 bp500 bp600 bp700 bp800 bp900 bp1,000 bp2. The DNA fragments from each subject contains one inherited allele. Therefore, there should betwo bands in each lane, one representing the allele inherited from the subject’s father and oneinherited from the subject’s mother.List the distances traveled by each band in each lane, along with estimates for their sizes basedon the DNA Ladder in the Answers below.Mother’s BandsDistance (mm) Size (bp)Band #1Band #2Child’s BandsDistance (mm) Size (bp)Band #1Band #2Alleged Father #1’s BandsDistance (mm) Size (bp)Band #1Band #2Alleged Father #2’s BandsDistance (mm) Size (bp)Band #1Band #23. The child should have inherited one of its alleles from her mother. Which band is it?Hint: Identify it by noting which band is the same bp for both mother and daughter.4. Is this band present in either of the alleged father’s DNA? If it were, how would that complicateidentifying the father?5. The child’s other band is the one that was inherited from her father. Which alleged father, if any,can be definitively ruled out from being the child’s biological father? This man is the one withneither band matching the child’s. Of the two men, is there one who is likely the father?6. Forensic scientists are trying to match suspects to a DNA sample left at the scene of a crime. Thesample from the crime scene has 900 bp and 260 bp bands. The scientists have DNA samplesfrom four suspects:o Suspect I has 900 bp and 540 bp bandso Suspect II has 840 bp and 260 bp bandso Suspect III has 900 bp and 260 bp bandso Suspect IV has 730 bp and 380 bp bands.Which suspect is most probably the criminal? 7. John Doe has two possible fathers. John Doe’s mother has 850 bp and 400 bp bands. John Doehas 680 bp and 400 bp bands. Possible Father I has 680 bp and 500 bp bands. Possible FatherII has 680 bp and 280 bp bands. Can you identify John Doe’s father?
Recent Comments