Solved by a verified expert:outline Final Applied Lab Project
Instructions
Outline (10 points) submit in this
assignment folder by deadline.
See syllabus for
detailed description and grading rubric for the final applied lab project
Include the following in your outline:
Name of enzyme you will use
Name of organism (if applicable)
The substrate
Method for measuring enzyme activity
Treatment: acidic fluid(s), pH, length of exposure,
how you will treat your samples
The control(s) in the experiment
Sample size
Hypothesis
How you will present your data (table and/or type
of graph)
Anything else you would like to get feedback on
before you start your experiment
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Final Applied Lab Project
Instructions
Final Applied Lab Project (1 credit Lab
Component)
Addresses course outcomes 1-4:recognize
and explain how the scientific method is used to solve problems
*make observations
and discriminate between scientific and pseudoscientific explanations
weigh
evidence and make decisions based on strengths and limitations of
scientific knowledge and the scientific
method
use
knowledge of biological principles, the scientific method, and
appropriate technologies to ask relevant
questions, develop
hypotheses, design and conduct experiments, interpret results, and draw
conclusions
This is the culminating lab assessment
in BIOL 103.It is designed to assess your ability to apply
the principles of the
scientific method.
The Effect of low pH on Enzyme Activity
Design an experiment in which you will test
the effect of an acidic fluid on enzymatic activity. Recall: enzymes are
proteins! To complete this project, it may
be useful for you to first review the Scientific Method Tutorial, the OLI
module
about pH and enzymes, Lab 1 (Introduction to
Science) and Lab 4 (Enzymes). As you review Lab 4, you will be reminded
that there are several factors that impact enzymatic activity: pH,
temperature, and amount of reagent. It is OK to use the same
enzyme/substrate/method as you did in lab 4 (but modify the treatment), or
you can search on-line to find a different enzyme/substrate/method for
measuring enzyme activity for your
project (include all references).
As you design your experiment for this
project, please remember that you are trying to examine how an acidic
fluid will
modify the outcome of an enzymatic
reaction. To successfully complete this project, you will need to
identify the
question(s) being asked in your experiment
and the hypothesis that you are testing. In your experimental design,
you must clearly explain what you are doing.
That means that you will need to identify the enzyme, the substrate, the
acidic fluid used as treatment, the control
treatment and the method of measuring enzyme activity, as well as explain
your experimental protocol. You must also thoroughly explain how the
acidic fluid impacted enzyme activity based on the results from your own
experiment
as well as knowledge of enzymes and pH from
the OLI modules, lab manual and potentially additional information
sources.
Hint: Keep in mind that the acid will change the environmental
conditions of the experiment (for example, a low pH
value could change the shape of the active
site on the enzyme protein), without directly participating in the reaction.
Lab Materials
You may need all or some of
the following, depending on your experimental design:
Materials from your lab kit:
pH
paper
hydrogen
peroxide solution (you can purchase this at a pharmacy if you have used
up the bottle that came with
the lab kit)
plastic
beakers or cups
vinegar
yeast
(can be purchased at grocery store if more is needed)
balloons
plastic
bottle
marker
for labeling of beakers
You may choose to use additional materials
(different acidic fluids and/or different organisms and/or different
substrate
if you chose an enzyme other than catalase).
Outline (10 points) submit in assignment folder in week 7.
Include the following in your outline:
Name
of enzyme you will use
Name
of organism (if applicable)
The
substrate
Method
for measuring enzyme activity
Treatment:
acidic fluid(s), pH, length of exposure, how you will treat your samples
The
control(s) in the experiment
Sample
size
Hypothesis
How
you will present your data (table and/or type of graph)
Anything
else you would like to get feedback on before you start your experiment
Write a paper that includes the following:
1. Title page: descriptive title, your name,
course name, semester
2. Introduction: general background
information about enzymes and specific information about your chosen enzyme,
the
question(s) that you are asking and a clear hypothesis for your experiment
(15 points).
3Design an experiment. Provide a detailed
description of the materials and methods used to conduct the experiment.
Identify control and experimental samples,
as well as independent and dependent variables. Also include the methods
used for data collection and analysis (20
points).
4. Conduct the experiment and record your
results. What did you observe? Present your data in table and/or graph
format. Remember to label everything and include the unit of measure with all
numbers (20 points)
5. Use your knowledge of enzymes and pH to
interpret and discuss your results. It may be necessary for you to refer to
the
OLI course modules, lab manual and/or use additional information resources.
What effect does the acidic treatment
have on enzyme activity? Did you get the
expected results? Explain. (15 points)
6. State a specific and accurate
conclusion. Is your hypothesis supported by the results? Looking back, how
could you
have improved your experiment? (10
points)
7. Include a list of references to all
information sources used in APA format (5 points).
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