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What property of a compound light microscope enables a person to change objectives without major focusing adjustments?
How does closing the iris diaphragm affect image brightness and contrast?
decreases brightness and increases contrast
increases brightness and decreases contrast
decreases brightness but has no effect on contrast
Which of the following objectives has the shortest working distance?
What effect will changing from the low power objective to the high power objective have on the field of view of a specimen?
the letter p is placed under the microscope in the normal reading
position, which of the following orientations of the letter would the
Which of the following procedures should be done just prior to adjusting the diopter, when using the compound microscope?
Clean all lenses with lens paper
Turn the light on
Adjust the iris diaphragm
Adjust the fine focus
Two parts of the microscope that regulate the amount of light visible through the ocular are the:
fine and coarse adjustment knobs
ocular and objective lenses
illuminator and revolving nosepiece
condenser and iris diaphragm
Initial focusing of any slide is done under the:
scanning or low power objective
high power objective
oil immersion objective
depends upon the size of the specimen
Under which magnification would the distance between the objective and the specimen being observed be the least?
What is the total magnification produced by a microscope, using a 10X ocular lens and a 10X objective lens?
Which of the following describes proper microscope care and technique.
Be sure to carry the microscope upright, with one hand on the arm and the other under the base.
To protect the optics of the microscope, place it down gently and don’t drag it across the tabletop.
Always begin focusing with the lowest power lens available.
Only use the coarse focus knob with the scanning and low power lenses.
All the above are examples of correct microscope care and technique.
Turning which of the following knobs moves the objective lenses the greatest vertical distance?
coarse focus knob
Resolution is the ability to see two objects as separate.
Which of the following regulates the amount of light passing through the slide specimen on the microscope stage?
iris diaphragm lever
fine focus knob
An increase in magnification causes
a decrease in the field of view.
a decrease in the working distance.
a decrease in the depth of field.
all of the above.
Working distance is the
distance from your house to your job.
distance the microscope nosepiece travels using the coarse focus knob.
distance from the bottom of the objective lens to the specimen.
distance the specimen can travel across the microscope stage.
adjusting a binocular microscope for differences between right and left
eyes, focus the microscope with the fine adjustment while viewing only
with the right eye (fixed ocular).
When using the oil immersion lens, you should raise the objective lenses for easy application of an oil droplet to the slide.
must a small object seen through the ocular lens be centered in the
field of view, before changing to a higher power objective lens?
to prevent damage to the objective lens
to compensate for the decrease in resolving power of the lens
to prevent its disappearance due to the decrease in the field of view
to prevent eyestrain
parfocal microscope allows you to quickly focus using the low power or
scanning lens and then, to switch to a higher power lens with only a
minimal adjustment of the fine focus knob.
Why is it necessary to adjust the amount of light after changing objective lenses?
Higher magnification lenses require more light.
Proper lighting is needed to see specimen details.
The lens aperture decreases with higher magnification lenses.
All of the above.
Which objective would you use to locate a specimen on a slide?
lowest power available
any of the ojectives could be used with equal success
Which of the following parts of the microscope would increase the depth of field?
high power objective
low power objective
Depth of field is best demonstrated with
a slide showing the letter e.
a slide containing overlapping threads.
the light turned completely off.
the oil immersion lens.
When the letter e slide is viewed with the microscope, it appears
right side up.
right side up and backwards.
upside down and backwards.