Solved by a verified expert:WEEK 8 EXPERIMENT ANSWER SHEET
Please
submit to the Week 8 Experiment dropbox no later than Sunday midnight.
SUMMARY
OF ACTIVITIES FOR WEEK 8 EXPERIMENT ASSIGNMENT
Experiment 8 Exercise 1 –Species
Interactions: Competition
Experiment 8 Exercise 2 – Biomes (Part I and II)
Experiment
8 Exercise 1: Species Interactions: Competition
In this
exercise you will be evaluating the effect of competition on the population size
of two species of microorganisms. Be
sure you have read through the readings for Week 8 as well as the introductory
information for the Week 8 Experiment. When you are ready to begin, open in the
following website:
Glencoe-McGraw
Hill. No date. Population Biology
http://glencoe.mcgraw-hill.com/sites/dl/free/0078759864/383928/BL_04.html
Procedure
A. Click on the Information
button on the bottom and read through the material before beginning. You will
need to scroll down to read all of the information. Close the window when you
are done. Note that the two species we will be using will be competing for the
same food source; bacteria.
B. First, you need to set up the
experiment by distributing the two species to the three test tubes.
a. Click on the pipette (the purple
bulb) in the flask containing P. caudatum,
fill it and place the contents in Tube #1.
b. Then click on the pipette in
the flask containing P. aurelia, fill
it and place the contents in Tube #2.
c. Finish by putting a pipette full of
both species in the Tube #3.
C. Answer the question below
before proceeding.
Question
1. The number of P. caudatum and P. aurelia
grown alone would be expected to increase until the population size reaches the
carrying capacity of the test tube. What do you think will happen in terms of
population growth in Tube #3 that contains both species combined and why (2
pts)?
Procedure (continued)
D. You now need to count the
number of organisms in each Tube beginning on Day 0 and continuing every
2 days until you reach Day 16. These values will need to be recorded in Table
1 below (do NOT use the Table provided by the website).
a. Click on the Microscope to
get started.
b. Click on Clean microscope slides
and then Take Sample.
c. Click on the first slide and
drag it on to the microscope. Count the number of P. caudatum(note its shape) and multiply by 2 to get the number of cells per
ml (your slide holds 0.5 ml). Record this number in the Table below; this is Day
0.
d. Next, click on the second slide
and drag it to the microscope. Count the number of P. aurelia (note its shape), multiply by 2 and record this number in the
Table for Day 0.
e. Finally, drag the third slide
on to the microscope and count the number of each type of organism, multiply
by 2 and enter the data into the Table.
f.
Click on
Clear Slides (on the bottom) and then on the Calendar that says
Day 0 to advance it two days.
g. Repeat steps b
– f until you reach 16 days.
h. As the days go on, you will have
more and more individuals to count. Click on the Grid On button
on the microscope to make them easier to count.
Table 1.Results(4 pts).
Grown Separately (cells per ml)
Grown Together (cells/ml)
Day
P. caudatum
P. aurelia
P. caudatum
P. aurelia
0
2
4
6
8
10
12
14
16
E. Now it is time to analyze
your data.
a. You will need to generate two graphs,
one which depicts the number of both species per day of culture when grown
separately and one that depicts the number of both species per day of
culture when grown together.
b. You must use the Scatter type
graph in Excel and each graph should have two lines (one for each species).
c. Be sure you label your axes and
your series; meaning you will need to indicate which line pertains to P. caudatum and which to P. aurelia.
Paste your two graphs below (4 pts):
Questions
2. What were the carrying
capacities (maximum population size) for the two species when grown
separately and on what day were they reached (1 pts)?
3. Describe what happened when the two
species were grown together and explain why. Be sure to discuss the
magnitude and timing of each species’ carrying capacity compared to when they
were grown separately (3 pts).
4. Do these results support the
principle of competitive exclusion; why or why not? Be sure to cite your sources. (4 pts).
Experiment 8 Exercise 2: Biomes
In these two relatively short exercises, we will be examining the
biotic and abiotic factors that define a biome. You should have completed the readings for this week before
beginning.
Procedure – Part I: The
Great Graph Match
A.
Open the following website:
NASA. No date. The Great Graph Match
http://earthobservatory.nasa.gov/Experiments/Biome/graphmatch_advanced.php
B.
In the Great Graph Match,
you will need to match abiotic
information (annual rainfall and temperatures) to the appropriate biome. Follow
the instructions on the page and fill-in the Table below. For the Explanation column, you need to briefly explain why you chose the biome
you did based on the data presented.
C.
Be sure to provide complete citations
for the sources used.
Table 2. Locations, biomes and
explanations (4 pts).
Location
Biome
Explanation
Frogmore, England
Goteborg, Sweden
Koombooloomba, Australia
Barrow, Alaska
Alice Springs, Australia
San Bernadino, California
Centralia, Kansas
Citations:
Procedure – Part II: To
Plant or Not to Plant
A.
Open the following website:
NASA. No date. To Plant or Not to Plant
http://earthobservatory.nasa.gov/Experiments/Biome/plant_it.php
B.
In the To Plant or not to
Plant, you will need to determine which in which biomes to plant various
plants, based on the information presented. Follow the instructions on the page
and fill-in the Table below. For the
Explanation column, you need to briefly
explain why you chose the biome you did.
C.
Be sure to provide complete citations
for the sources used.
Table 3. Plants, biomes and
explanations (4 pts).
Plant
Biome
Explanation
Creosote bush
Spruce
Flowering dogwood
Orchid
Lichen
Bluestem grasses
White sage
Saguaro cactus
Citations:
Week 8 Experiment
Grading Rubric
Component
Expectation
Points
Experiment 8 Exercise 1
Correctly conducts experiment, records data (Table 1) and
generates two graphs.
8 pts
Experiment 8 Exercise 1
Demonstrates an understanding competition between species and
the effect it has on population growth (Questions 1-4).
10 pts
Experiment 8 Exercise 2
Develops an appreciation of the diversity of ecosystems around
the globe and understands the environmental conditions that lead to their
development (Tables 2 and 3).
8 pts
TOTAL
26 pts
Recent Comments