Solved by a verified expert:BIO 460 Exam
I (25% each)

1.
Assume that a 4000-bp long circular DNA plasmid has two HindIII sites at coordinate 1 and 801. If we digest this circular DNA plasmid with BamHI, we obtain 500 bp, 1400 bp, and
2100 bp DNA fragments. If we perform HindIII/BamHI double digestion with this circular plasmid, we obtain 200
bp, 500 bp, 600 bp, 1200 bp, and 1500 bp DNA fragments. From above information, please interpret the
location of BamHI sites.
(1) How many BamHI sites are there in this circular
plasmid?
(2) Where are those BamHI sites? Please draw the map.

2.
Design the primers to clone the specific gene into the vector as indicated to
make the fusion protein with FLAG tag.
You will need to show the Tm, primers, restriction enzymes, and
recombinant DNA molecule. Please follow
the instruction to find your project.

pESCTRP ESA1 (your last digit of SS# is 0
&1)

pESCHIS INO80
(your last digit
of SS# is 2 &3)

pESCLEU HHT1
(your last digit of SS# is 4
&5)

pESCHIS HHO1
(your last digit of SS# is 6
&7)

pESCLEU HHF1 (your
last digit of SS# is 8 &9)

3. Please describe one
example (including the experiments) to demonstrate that the H1 may be repressor
of specific genes.

4. Please describe one
example (including the experiments) to demonstrate that the histone acetylation
is important to transcriptional activation.