Solved by a verified expert:Molecular lab we have been given the opportunity to propose a molecular assay and
develop a molecular laboratory. This
will require that we write a proposal to administration. This proposal must include our assay design
and equipment list. we must propose how
we would use the available space given the included floor plan. Each section of
this exercise will require that we adhere to the space limitations.

1. ONE PAGE MAXIMUM

The gene we will need to design a test around is
provided. The polymorphism is noted:

5′
301 gtcagcccca tggtggtggc tggggacagc cacatggtgg tggaggctgg
ggtcaaggtg

361 gtagccacag tcagtggaac aagcccagta agccaaaaac caacaygaag
catgtggcag

421 gagctgctgc agctggagca gtggtagggg gccttggtgg ctacatgctg
ggaagtgcca

3′
481 tgagcaggcc tcttatacat tttggcaatg actatgagga ccgttactat
cgtgaaaaca

Describe in detail the assay we will design. Be as specific as possible. No make believe enzymes or reagents. Nothing magical.
At a minimum our description should include:
-A diagram
indicating where any primers or probes would anneal to
the above sequence (we can annotate the
sequence above).
-A list of any
reagents needed, i.e. oligonucleotides, enzymes, etc.
-A statement of
how we will detect the polymorphisms
-Controls needed
-The
concentrations and formulations of any gels used
-The
concentrations and components of any enzymatic reactions used
-A diagram of how
the results of our assay would appear including
controls, a
homozygous individual and a heterozygote.

Priniciple of test:

Reagents needed:

Controls needed:

Outline of assay:

Diagram of results:

2. ONE PAGE MAXIMUM

Given the
following floor plan, indicate how we would assign the activities related to
our assay. describe how we would control contamination. At a minimum it is expected that we will
include areas for:
Specimen
processing
Data Analysis
Pre-PCR
Post-PCR
Reagent
preparation
Storage
Darkroom
Show where
refrigerators, freezers, thermocyclers, safety equipment, hoods and other
equipment.

indicate traffic flow if it is integral to our contamination
control plan.

we are not allowed to add, move or remove walls. we can add doors if we feel it necessary

1/2″ = 5 ft