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Final Applied Lab Project
Instructions
Final
Applied Lab Project
Addresses course outcomes
1-4:
recognize and explain how the
scientific method is used to solve problems
make observations and
discriminate between scientific and pseudoscientific explanations
weigh evidence and make
decisions based on strengths and limitations of scientific knowledge and
the scientific method
use knowledge of biological
principles, the scientific method, and appropriate technologies to ask
relevant questions, develop hypotheses, design and conduct experiments,
interpret results, and draw conclusions
This is the culminating lab
assessment in BIOL 103. It is designed to assess your ability to apply the
principles of the scientific method.
The Effect of low pH on Enzyme
Activity
Design an experiment in which you
will test the effect of an acidic fluid on enzymatic activity. Recall:
enzymes are proteins! To complete this project, it may be useful for you to
first review the Scientific Method Tutorial, the OLI module about pH and
enzymes, Lab 1 (Introduction to Science) and Lab 4 (Enzymes). As you
review Lab 4, you will be reminded that there are several factors that impact
enzymatic activity: pH, temperature, and amount of reagent. It is OK to
use the same enzyme/substrate/method as you did in lab 4 (but modify the
treatment), but you are encouraged to search on-line to find a different
enzyme/substrate/method for measuring enzyme activity for your project
(include all references).
As you design your experiment for
this project, please remember that you are trying to examine how an acidic
fluid will modify the outcome of an enzymatic reaction. To
successfully complete this project, you will need to identify the question(s)
being asked in your experiment and the hypothesis that you are testing.
In your experimental design, you must clearly explain what you are doing.
That means that you will need to identify the enzyme, the substrate, the
acidic fluid used as treatment, the control treatment and the method of
measuring enzyme activity, as well as explain your experimental
protocol. You must also thoroughly explain how the acidic fluid
impacted enzyme activity based on the results from your own experiment as
well as knowledge of enzymes and pH from the OLI modules, lab manual and
potentially additional information sources.
Hint: Keep in mind that the acid will change the
environmental conditions of the experiment (for example, a low pH value could
change the shape of the active site on the enzyme protein), without directly
participating in the reaction.
Lab Materials
You may need all
or some of the following, depending on your experimental design:
Materials from your lab kit:
pH paper
hydrogen peroxide solution
(you can purchase this at a pharmacy if you have used up the bottle that
came with the lab kit)
plastic beakers or cups
vinegar
yeast (can be purchased at
grocery store if more is needed)
balloons
plastic bottle
marker for labeling of
beakers
You may choose to use additional
materials (different acidic fluids and/or different organisms and/or
different substrate if you chose an enzyme other than catalase).
Outline (10 points) submit in assignment folder in week 7.
Name of enzyme you will
use:
Name of organism (if
applicable):
The substrate:
Method for measuring enzyme
activity:
Treatment: acidic fluid(s),
pH, length of exposure, how you will treat your samples: The control(s) in the experiment:
Sample size:
Hypothesis:
How you will present your
data (table and/or type of graph):
Write a lab report that includes
the following:
1. Title page: descriptive title,
your name, course name, semester
2. Introduction: general
background information about enzymes and specific information about your
chosen enzyme, the question(s) that you are asking and a clear hypothesis for
your experiment (15 points).
3. Design an experiment. Provide a
detailed description of the materials and methods used to conduct the
experiment. Identify control and experimental samples, as well as independent
and dependent variables. Also include the methods used for data collection
and analysis (20 points).
4. Conduct the experiment and
record your results. What did you observe? Present your data in table
and/or graph format. Remember to label everything and include the unit of
measure with all numbers (20 points)
5. Use your knowledge of enzymes
and pH to interpret and discuss your results. It may be necessary for you to
refer to the OLI course modules, lab manual and/or use additional information
resources. What effect does the acidic treatment have on enzyme
activity? Did you get the expected results? Explain. (15 points)
6. State a specific and
accurate conclusion. Is your hypothesis supported by the results? Looking
back, how could you have improved your experiment? (10 points)
7. Include a list of references to
all information sources used in APA format (5 points).
Avoid Plagiarism
It is very important to write
with your own words. If you do copy one or two sentences directly
(use sparingly), use quotation marks (“) around the copied text,
followed by an in-text reference. All information sources need to be included
in the reference list and as in-text references. Plagiarism will be reported
to the proper UMUC authority. Guidance on how to avoid plagiarism can be
found here:UMUC’s How to Avoid Plagiarism
Submission
Submit your final applied lab
project to both the TurnItIn website as well as in the assignment folder by
the due date specified in the course schedule.
Grading
Your Final Applied Lab Project
will be graded based on the following criteria (100 points total):
Hide RubricsRubric
Name: Final Applied Laboratory Project
Criteria
Excellent (90-100%)
100 points
Good (75-89%)
89 points
Adequate (50-74%)
74 points
Unacceptable (0-49%)
49 points
Outline
10 points (Due in week 7)
Outline includes all the
required components and planned experiment meets requirements for the
assignment and is clearly and accurately described. Submitted on time.
9-10 points
Outline missing one or two of
the required components, and/or planned experiment does not meet one
of requirements for the assignment and/or minor issues with clarity
and accuracy.
7-8 points
Outline missing several of the
required components, and/or planned experiment does not meet several
of the requirements for the assignment and/or major issues with
clarity and accuracy.
5-6 points
Outline missing most of the
required components andplanned experiment does not meet the
requirements of the assignment.
0-4 points
Introduction,
Question and Hypothesis (15 points)
Background information about
enzymes in general and about specific enzyme used in project is clearly and
accurately written. Questions and hypothesis are specific, relevant and
clearly stated.
14-15 points
Background information about
enzymes in general and about specific enzyme is somewhat unclear and/or
inaccurate. Questions and hypothesis could be more specific, relevant and
clearly stated.
11-13 points
Missing background information
about enzymes in general or about specific enzyme used in project or
questions and hypothesis.
7-12 point
Missing background information
about enzymes in general and about specific enzyme used in project and
questions/hypothesis.
0-6 points
Experimental
Design, Materials and Methods (20 points)
Experiment is designed to directly
test the hypothesis; description of experiment is detailed and well written
and includes all materials and methods used.
18-20 points
Experiment is mostly designed to
test the hypothesis and/or description of experiment is somewhat
inaccurate and/or some information about materials and methods used
is missing and/or minor problems with clarity an organization.
15-17 points
Experiment is barely designed to
test the hypothesis and/or description of experiment is inaccurate and/or
most of the materials and methods used are missing and/or major
problems with clarity an organization.
10-14 points
Experiment is not designed to
test hypothesis and/or description of experiment and materials and
methods used are missing.
0-9 points
Results
(20 points)
Results are clearly and
accurately presented in a table and/or graph format.
18-20 points
Results are presented, but minor
problems with clarity and/or accuracy.
15-17 points
Results are described, but major
problems with clarity and/or accuracy and/or results not presented
in table or graph.
10-14 points
Results are not included
0-9 points
Discussion
of Results (15 points)
A clear, accurate and well
organized discussion of results that demonstrates good knowledge of
enzymes.
14-15 points
Minor problems with clarity,
accuracy or organization and/or demonstrates some gaps in knowledge
of enzymes.
15-17 points
Major problems with clarity,
accuracy or organization or demonstrates minimal knowledge of
enzymes.
10-14 points
Missing discussion of results,
or major problems with clarity, accuracy or organization andmissing
understanding of enzymes.
0-9 points
Conclusion
( 10 points )
A well stated conclusion that is
accurate and specific and directly related to the data collected. Clearly stated
whether or not the results support the hypothesis. Relevant suggestion for
improvement of experiment.
9-10 points
Conclusion stated, but slightly
inaccurate, and/or not directly related to data collected and/or
suggestion for improvement of experiment not very relevant.
7-8 points
Conclusion stated, but
inaccurate and/or barely related to data collected and/or
missing suggestion for improvement of experiment.
5-6 points
Missing conclusion, only
suggestion for improvement of experiment.
0-4 points
Citations
(5 points)
All references used are included
and in correct APA format, in-text references are included.
5 points
All references are included, but
a few minor mistakes in APA format, and/or missing in-text
references.
4 points
Missing one or several
references and/or major mistakes in APA format.
3 point
No references included or
references not in APA format.
0-2 points
Grammar
and Spelling (5 points)
Less than 3 minor
spelling and grammatical errors
5 points
Between 4 and 7 minor spelling
and grammatical errors
4 points
Between 8 and 12 spelling and
grammatical errors
3 point
More than 13 spelling and
grammatical errors
0-2 points
Overall Score
Level 4
90 or more
Level 3
75 or more
Level 2
50 or more
Level 1
0 or more
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